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. Author manuscript; available in PMC: 2017 Dec 15.
Published in final edited form as: Arch Biochem Biophys. 2016 Oct 18;612:46–56. doi: 10.1016/j.abb.2016.10.011

Figure 2. Oxidation of Dihydronicotinamide Nucleosides (NR) and Mononucleotide (NMN) Substrates by Renalase.

Figure 2

NR+ and NMN+ were reduced by the addition of sodium borohydride forming three reduced isomers of each. These mixtures were then reacted with renalase (12.5 µM) for 10 minutes. A and C are HPLC chromatograms that showed the three species formed by reduction without (black trace) and with (red trace) the incubation with renalase. B and D are the extinction coefficient spectra for each of the reduced forms obtained, each corresponding to the chromatograms shown to the left.