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. Author manuscript; available in PMC: 2017 Dec 15.
Published in final edited form as: Arch Biochem Biophys. 2016 Oct 18;612:46–56. doi: 10.1016/j.abb.2016.10.011

Figure 3. Reductive Half Reactions with 2- and 6Dihydro- NMN and NR with and without complementary ligands.

Figure 3

A. Representative reduction traces for HsRen (10 µM) reacting with 6DHNR in the presence (black) and absence (grey) of 4 mM ADP. Data were fit to a single exponential according to equation 1. Lines of best fit over the data (blue). B. Secondary plot showing the effect of added ADP on the observed rate constant for reduction of HsRen by both 2- and 6DHNAD. Data were fit to equation 2 to determine values for Kred/Kd from the slope term.