BMP2, CK2.2, and CK2.3 but not CK2.1 induced chondrocyte hypertrophy and mineralization of C3H120T1/2 cells. C3H10T1/2 cells cultured for 3 weeks and stimulated with CK2.1 (100 nM or 500 nM), or 100 nM of CK2.2 or CK2.3 and stimulated or not with the equivalent concentration of BMP2 (40 nM or 200 nM) as controls. Cells were fixed and then stained for Hoechst (blue) (A), collagen type × (green) (B), osteocalcin (green) (C), and von Kossa and analyzed for results using ImageJ. Scale bar representing 50 µm.