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. 2017 Jul 24;8:833. doi: 10.3389/fimmu.2017.00833

Figure 2.

Figure 2

Effect of an intramammary injection with lipopolysaccharide (LPS) and lipoteichoic acid (LTA) on neutrophil recruitment in the intraductal mouse model. (A) Schematic presentation of the set-up. The white mammary glands represent the PBS (sham)-inoculated mammary glands, whereas the patterned glands represent the glands inoculated with LPS or LTA. (B) IL-8, a prototype chemoattractants for PMN influx was determined in mammary gland tissue 24 h p.i. of different doses of LPS and LTA (for inoculum dose 0.01, 0.1, 1, 10, and 10,000 EU LPS/LTA: n = 3 mice/6 glands; for inoculum dose 100 and 1,000 EU LPS/LTA: n = 2 mice/4 glands). Represented data were pooled from two independent experiments. Values are expressed as mean ± SEM. Significance between LPS-treated glands and PBS (sham)-inoculated mammary glands or significance between LTA-treated glands and PBS (sham)-inoculated mammary glands is illustrated without brackets. Values are expressed as mean ± SEM. **P < 0.01 and ***P < 0.001 versus sham. Significance between LPS- and LTA-inoculated glands is illustrated with brackets. Values are expressed as mean ± SEM. ***P < 0.001. (C) Mammary tissue was evaluated histologically p.i. with different doses of LPS or LTA. Immune cells in the alveolar lumen are indicated with black arrows and enlarged in the images below. Scale bar, 100 µm. p.i., post-inoculation.