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. Author manuscript; available in PMC: 2017 Jul 24.
Published in final edited form as: Discov Med. 2013 Jan;15(80):7–15.

Figure 4.

Figure 4

Identification of IKKβ phosphorylation sites that are critical for β-TRCP-mediated destruction of Twist. Immunoblot analysis of HeLa cells transfected with the indicated HA-Twist and Flag-β-TRCP plasmids in the presence or absence of Flag-IKKβ. A plasmid encoding GFP (green florescent protein) was used as an internal control for transfection efficiency. 7A, S7A/S8A/S11A/S16A/S20A/T125A/S127A.