Figure 4.
Identification of IKKβ phosphorylation sites that are critical for β-TRCP-mediated destruction of Twist. Immunoblot analysis of HeLa cells transfected with the indicated HA-Twist and Flag-β-TRCP plasmids in the presence or absence of Flag-IKKβ. A plasmid encoding GFP (green florescent protein) was used as an internal control for transfection efficiency. 7A, S7A/S8A/S11A/S16A/S20A/T125A/S127A.