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. 2017 Jul 12;174(16):2784–2796. doi: 10.1111/bph.13904

Figure 5.

Figure 5

Changes in cAMP responses in different subcellular domains following EP2 receptor stimulation. (A–D) Representative time course of changes in the magnitude of the normalized FRET response (ΔR/R0) in cells expressing Epac2‐MyrPalm (MyrPalm), Epac2‐CAAX (CAAX), Epac2‐NLS (NLS) and Epac2‐camps (Epac2), under control conditions, and following exposure to 0.3 and 1 μM butaprost (But). (E) Size of average FRET responses to butaprost. The responses to 0.3 μM butaprost detected by MyrPalm (n = 11) and Epac2 (n = 8) were significantly smaller than those detected by CAAX (n = 12) and NLS (n = 7). The response to 1 μM butaprost detected by MyrPalm (n = 11) was significantly smaller than the responses detected by CAAX (n = 10), NLS (n = 7) and Epac2 (n = 8). The Epac2 response was also significantly smaller than the NLS response. Statistical significance was tested using one‐way ANOVA followed by Holm‐Sidak method for pairwise multiple comparisons. All responses were normalized to the maximal responses elicited by 10 μM FSK plus 100 μM IBMX.