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. 2017 Jul 12;174(16):2784–2796. doi: 10.1111/bph.13904

Figure 8.

Figure 8

Effect of AC2 overexpression on cAMP responses elicited by β‐adrenoceptor stimulation. Representative time course of changes in the normalized FRET response (ΔR/R0) detected by Epac2‐MyrPalm (MyrPalm) (A) and Epac2‐CAAX (CAAX) (B), following exposure to 1 and 3 nM isoprenaline (Iso) in cells overexpressing AC2. (C) Size of average FRET responses in AC2 overexpressing (OE) cells; 1 nM Iso: MyrPalm (n = 5); CAAX (n = 3); 3 nM Iso: MyrPalm (n = 5); CAAX (n = 3). (D) Difference in magnitude of Iso responses due to AC2 overexpression; 1 nM Iso: MyrPalm (n = 13); CAAX (n = 11); 3 nM Iso: MyrPalm (n = 13); CAAX (n = 11). There was no significant difference in the effects of AC2 overexpression on the responses detected by the different biosensors (one‐way ANOVA). All responses were normalized to the maximal response elicited by 1 μM Iso plus 100 μM IBMX.