Table 2.
Kinetic parameters of functional turnover for the recovery of ME (3.2 μM, 1 min)‐induced inhibitory effect after different exposure times to β‐FNA (300 nM, 15–60 min)
| β‐FNA 300 nM | ||||
|---|---|---|---|---|
| 15 min (n = 6) | 30 min (n = 8) | 45 min (n = 5) | 60 min (n = 5) | |
| ME effect (%) | ||||
| Control | 100 ± 0.0 | 99.4 ± 0.4 | 100 ± 0.0 | 88.5 ± 9.1 |
| t 15–60 = 0 min | 14.7 ± 3.3 | 5.5 ± 2.6 | 8.5 ± 2.3 | 5.0 ± 3.6 |
| t 15–60 = 120 min | 84.0 ± 4.4 | 58.6 ± 5.4* | 31.0 ± 7.1* | 35.4 ± 3.9* |
| Functional turnover of ME effect | ||||
| r′ (Emax · min−1) | 2.52 ± 0.64 | 1.78 ± 0.29 | 1.04 ± 0.34 | 0.81 ± 0.22* |
| k′ (min−1) | 0.026 ± 0.006 | 0.031 ± 0.006 | 0.025 ± 0.009 | 0.021 ± 0.005 |
| t ½ ′ (min) | 33 ± 6 | 32 ± 8 | 40 ± 10 | 46 ± 13 |
| E ss (%) | 97.3 ± 6.4 | 62.0 ± 5.7* | 45.1 ± 11.6* | 39.5 ± 4.67* |
Values are expressed as mean ± SEM obtained by nonlinear regressions of n cells (see the Methods section). Parameters: r′ and k′ are the reappearance and disappearance rate constants of ME effect, t ½ ′ is the t 1/2 of ME effect and E ss is the maximal recovery of ME effect at steady state.
*
P < 0.05, when compared with β‐FNA (15 min) by a one‐way ANOVA followed by a post hoc Bonferroni's multiple comparison test.