Fig. 2.

Brefeldin A inhibits primary cilium disassembly.

(A) Structural elucidation of KY-0120. The structure of KY-0120 was defined by NMR analyses (See Supplementary Fig. S1 for details). (B–F) hTERT-RPE cells serum-depleted for 48 h were treated with either the DMSO control, BFA, or menensin, 30 min prior to serum-restimulation. Cells were fixed at 24 h after serum-restimulation and subjected to immunocytochemistry. The cells with primary cilia were counted (C, F) and the lengths of primary cilia were measured (E). Data are mean ± SD from three independent experiments. >300 cells were counted for each sample. Statistical analysis: ***P < 0.001 (unpaired two-tailed t-test).