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. 2017 Jul 1;7(7):1407–1422.

Figure 6.

Figure 6

The overexpression of FOXO3a activates the p53-independent intrinsic apoptosis pathway. A and B: The overexpression of FOXO3a activates the downstream gene expression of PUMA. The mRNAs from MG63-R12, MG63-R12+FOXO3a, U2OS-R5, and U2OS-R5+FOXO3a cells were used to determine the expression of p53, PUMA, Bcl-2, Bax, Caspase-9, Caspase-3, Apaf-1 and FOXO3a. The relative expression of these genes in cells overexpressing FOXO3a was normalized against the individual gene expression in their corresponding non-overexpression cells (*P < 0.05, **P < 0.001). C: The overexpression of FOXO3a increases the protein levels of PUMA downstream. Cell lysates from MG63-R12, MG63-R12+FOXO3a, U2OS-R5, and U2OS-R5+FOXO3a cells were subjected to Western blot analyses for p53, PUMA, Bcl-2, Bax, Caspase-9, Caspase-3, Apaf-1 and FOXO3a. D: The overexpression of FOXO3a decreases the ability to form tumors in vivo. MG63-R12, MG63-R12+FOXO3a, U2OS-R5, and U2OS-R5+FOXO3a cells were injected intradermally into the flanks of mice. Tumor volumes were measured with fine calipers at 5-day intervals.