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. 2017 Jul 1;7(7):1463–1475.

Figure 2.

Figure 2

DGCR5 inhibited proliferation, migration and invasion of LC cells. A. The relative expression of DGCR5 was detected by qRT-PCR in human lung epithelial cells (BEAS-2B) and LC cell lines (H520, H157, SKMES1, H460, A549, and H1299, *P < 0.05, **P < 0.01). B. H1299 cells were transduced with GFP-expressing lentivirus (Lenti-GFP, control) and DGCR5 lentivirus (Lenti-DGCR5). H520 cells were transduced with shLuc (lentiviral vector-mediated shRNA against Luc, control) and shDGCR5 (lentiviral vector-mediated shRNA against DGCR5). Then, qRT-PCR was used to detect the expression level of DGCR5 (***P < 0.001). C. MTT assay was performed to detect the proliferative abilities of the transfected H520 and H1299 cells (***P < 0.001). D. The clonogenic capacities were measured by the colony formation assay in the transfected H520 and H1299 cells (***P < 0.001). E. Transwell assays were performed to analyze the migration and invasion abilities of H1299 cells transduced with Lenti-GFP or Lenti-DGCR5 and H520 cells transduced with shLuc or shDGCR5 (Magnification, × 200, ***P < 0.001).