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. 2017 Jul 1;7(7):1463–1475.

Figure 6.

Figure 6

DGCR5 decreased the expression levels of AKT, GSK-3β, and β-catenin through miR-1180. (A) The mRNA expression levels of AKT, GSK-3β, and β-catenin were analyzed by qRT-PCR in H1299 cells transfected with Lenti-GFP, Lenti-DGCR5, Lenti-DGCR5 and mimics NC or Lenti-DGCR5 and miR-1180 mimics (***P < 0.001). (B) The expression levels of AKT, GSK-3β, and β-catenin were detected by qRT-PCR in H520 cells transfected with shLuc, shDGCR5, shDGCR5 and inhibitors NC or shDGCR5 and miR-1180 inhibitors (***P < 0.001). The protein levels of AKT, p-AKT, GSK-3β, and β-catenin were measured by Western blotting in transfected H1299 (C) and H520 cells (D). GAPDH was used as the loading control.