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. 2017 May 15;6(5):e332. doi: 10.1038/oncsis.2017.30

Figure 4.

Figure 4

Mechanism of action of JQ1 in NETs. RNA sequencing (RNA-Seq) was performed in treated (JQ1) and control (JQ1-, untreated and DMSO) human NET cell lines BON-1, H727 and H720 and changes in gene and protein expression were assessed by qRT-PCR and Western blot analysis, respectively. (a) Expression of significantly dysregulated histone genes detected by RNA-Seq was examined by qRT-PCR. Untreated (UT) is represented by a black bar, DMSO control treatment by chequered bars, JQ1- control treatment by lined bars, and JQ1 by white bars. A one-way ANOVA, was used for statistical analsyis, *P<0.05; **P<0.005; ***P<0.0005. Data is represented relative to untreated cells. (b) Western blot analysis of H2B protein expression in JQ1 and control treated BON-1, H727 and H720 cells. GAPDH was used as a loading control. (c) Quantification of H2B Western blot analsyis using densitometry. Control treatments are represented by black bars and JQ1 treatment by white bars. One-way ANOVA using a Bonferroni correction for multiple comparisons was used for statistical analysis,*P<0.05; **P<0,005. Data and significance is represented relative to UT cells.