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. 2017 May 22;6(5):e339. doi: 10.1038/oncsis.2017.31

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Copyright © 2017 The Author(s)

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Sp1 regulates CYP17A1 expression. (a) The correlation of Sp1, NFIC, Sp3 and GATA6 mRNA with CYP17A1 mRNA in glioma was analyzed in the GSE4290 database. The number in x- and y-axes represent the expression value of gene in complementary DNA microarray. (b) The CYP17A1 promoter sequence and putative Sp1-binding sites are indicated in red. (c, d) After Sp1 overexpression or knockdown (e, f) U87MG cells were collected and subjected to western blotting with an anti-CYP17A1 antibody (left panel) and reverse transcription-quantitative PCR using primers against CYP17A1 (right panel). Sc: cells were infected by lentiviruses expressing scramble short hairpin RNA for control group; shSp1: cells were infected by lentiviruses expressing short hairpin RNA targeting Sp1. (g) Effects of Sp1 on different truncated promoters of CYP17A1. After transfection with the indicated plasmids for 24 hrs, the protein lysates were collected and subjected to reporter assays. Data are expressed as the means±s.e.m. (*P<0.05, ***P<0.001). NS, not significant.