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. 2017 May 22;6(5):e339. doi: 10.1038/oncsis.2017.31

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Copyright © 2017 The Author(s)

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Increased Sp1-binding to the CYP17A1 promoter in TMZ-resistant glioma cells. (a) Cells were subjected to CHIP assays, and the indicated promoter fragment was amplified by PCR. (b) The CYP17A1 promoter sequence contains two putative Sp1-binding sites. (c) Sp1-binding activity was determined by DAPA. After incubation of protein lysates with the biotinylated Sp1-binding sequence (S1 or S2 probe) and streptavidin beads, the beads were analyzed by western blotting with an anti-Sp1 antibody. (d) Quantitative results for Sp1 expression and Sp1-binding activity. Data are expressed as the means±s.e.m. (*P<0.05, **P<0.01, ***P<0.001). CHIP, chromatin immunoprecipitation; DAPA, DNA affinity precipitation assay.