Skip to main content
. 2017 May 15;6(5):e333. doi: 10.1038/oncsis.2017.32

Figure 5.

Figure 5

AR signalling alters PHB isoelectric charge point. (a) Western blot of PHB associated with the chromatin in LNCaP, VCaP and PC3 cells grown in media with charcoal-stripped serum for 72 h and then treated with ethanol or R1881 (10 nM) for 4 h. (b) Densitometry data for PHB (upper panel) and AR (lower) association with the chromatin in PC3, VCaP and LNCaP cells grown in media with charcoal-stripped serum for 72 h and then treated with ethanol or R1881 (10 nM) for 4 h. (c) 2D western blot for PHB from LNCaP cells grown in media with full serum or charcoal-stripped serum for 72 h. Blot represents isoelectric focussing of pH 3–10 horizontal and protein size vertical (4–12% gradient gel). (d) 2D western blot of PHB from LNCaP cells grown in media with charcoal-stripped serum for 72 h and then treated with ethanol or 10 nM R1881, or R1881 together with 10 μM enzalutamide for 4 h. Blot represents isoelectric focussing of pH 3–10 (horizontal) and protein size (vertical 4–12% gradient gel). Insert figure shows magnification and arrow indicates additional basic PHB species. (e) PC3 cells grown in media with charcoal-stripped serum for 72 h and then treated with ethanol or R1881 (10 nM) for 4 h. Blot represents isoelectric focussing of pH 3–10 (horizontal) and protein size (vertical 4–12% gradient gel). (f) 2D western blot of PHB from LNCaP cells transfected with GFP-tagged PHB, with isoelectric focussing pH 3–10 and linear 12% acrylamide gel.