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. 2017 May 8;6(5):e329. doi: 10.1038/oncsis.2017.34

Figure 5.

Figure 5

Glucose depletion increases ME1-dependent glutamine metabolism and reduces expression of NADPH-producing enzymes other than ME1 in HCT116 cells. (a) HCT116 cells were cultured in glucose-depleted or -limited media (2, 1, 0.5 or 0.2 g/l glucose or glucose-free) for 72 h. Cell viability was determined at 24, 48, and 72 h using CellTiter Glo. Relative cell growth, normalised by cell viability at Day 0, is shown in the line graph (n=3, t-test; *P<0.01). (b) HCT116 cells were cultured in media with or without glucose for 24 h and labelled with [U-13C, U-15N] l-glutamine isotope for an additional 24 h. Then, isotope profiling analysis was conducted. Percentage of the pool of metabolites and isotopes is shown in the bar graph (2 g/l glucose in black and glucose-free in white; n=3, mean with s.d.). (c) HCT116 cells were cultured in media with 2, 1, 0.5 or 0.2 g/l glucose for 72 h and mRNA expression levels of NADPH-producing enzymes at 0, 24, 48 and 72 h were determined by TaqMan PCR and are shown in the line graph (n=3).