Skip to main content
. 2017 May 15;6(5):e335. doi: 10.1038/oncsis.2017.37

Figure 4.

Figure 4

MiR-645 prevents CRC cells undergoing apoptosis. (a) WiDr and EB cells transduced with the miR-645 inhibitors or the control were pretreated with z-VAD-fmk (30 μM) for 1 h followed with the treatment of CDDP (5 μg/ml) or 5-FU (5 mg/ml) for an additional 48 h. Apoptosis was quantitated. mean±s.e., n=3. *P<0.05, Student's t-test. (b) Whole-cell lysates of WiDr and EB cells transduced with the miR-645 inhibitors or the control followed with the treatment with CDDP (5 μg/ml) or 5-FU (5 mg/ml) for 24 h were subjected to Western blot analysis, n=3. (c) WiDr and EB cells with miR-645 inhibition were transfected with the vector alone (pCMV6-AC-GFP-empty) or Mcl-1-GFP cDNA (pCMV6-AC-GFP-Mcl-1). Whole-cell lysates from were subjected to western blot analysis, n=3. (d) WiDr and EB cells with miR-645 inhibition were transfected with the vector alone or Mcl-1-GFP cDNA. Twenty-four hours later, cells were then treated with CDDP (5 μg/ml) or 5-FU (5 mg/ml) for a further 48 h. Apoptosis was quantitated. mean±s.e., n=3. *P<0.05, Student's t-test.