Table 1. Extracellular proteins whose expressions were regulated by 4α-PDD in a TRPV4-specific manner, that is, proteins that showed PDD-induced changes in control siRNA transfected but not in TRPV4-knockeddown cells.
| Mode of secretion | Fold change | MW (kDa) | % Coverage | P-value | |
|---|---|---|---|---|---|
| TRPV4-specific proteins downregulated following PDD treatment | |||||
| Gene_Symbol=Spp1 Osteopontin | Classical | 0.68 | 36 | 76.19 | 3.26E−14 |
| Gene_Symbol=Mtpn Myotrophin | Unconfirmed | 0.75 | 14 | 49.15 | 1.17E−02 |
| Gene_Symbol=B2m Beta-2-microglobulin | Classical | 0.71 | 14 | 60.50 | 4.00E−06 |
| Gene_Symbol=Clu Clusterin | Classical | 0.70 | 54 | 76.34 | 1.47E−04 |
| Gene_Symbol=Tinagl1 Tubulointerstitial nephritis antigen-like | Classical | 0.73 | 56 | 26.39 | 1.71E−02 |
| Gene_Symbol=E330026B02Rik Isoform 1 of Collagen alpha-6(VI) chain | Classical | 0.74 | 142 | 18.94 | 4.97E−02 |
| Gene_Symbol=Fn1 Putative uncharacterized protein | Classical | 0.77 | 297 | 54.34 | 3.36E−02 |
| Gene_Symbol=Atp5b ATP synthase subunit beta, mitochondrial | Exosome | 0.73 | 63 | 53.50 | 3.23E−02 |
| Gene_Symbol=Igfbp4 Insulin-like growth factor-binding protein 4 | Classical | 0.74 | 30 | 66.93 | 4.82E−02 |
| Gene_Symbol=Psap Sulfated glycoprotein 1 | Unconfirmed | 0.73 | 67 | 33.75 | 5.18E−03 |
| Gene_Symbol=Filip1 Filamin A interacting protein 1 | Unconfirmed | 0.61 | 138 | 32.21 | 9.42E−03 |
| TRPV4-specific proteins upregulated following PDD treatment mode of secretion | |||||
| Gene_Symbol=Gm1821 Putative uncharacterized protein | Unconfirmed | 2.12 | 18 | 99.35 | 6.37E−07 |
| Gene_Symbol=Dbi Acyl-CoA-binding protein | Unconfirmed | 1.36 | 10 | 73.56 | 2.17E−06 |
| Gene_Symbol=Nme2 Nucleoside diphosphate kinase B | Exosome | 1.34 | 18 | 96.05 | 1.24E−02 |
| Gene_Symbol=Psme1 Proteasome activator complex subunit 1 | Exosome | 1.43 | 30 | 57.03 | 1.85E−02 |
| Gene_Symbol=Asns Asparagine synthetase [glutamine-hydrolyzing] | Exosome | 1.42 | 67 | 31.55 | 2.49E−02 |
| Gene_Symbol=ENSMUSG00000072940; Rps28 40 S ribosomal protein S28 | Unconfirmed | 1.38 | 8 | 52.17 | 3.40E−02 |
| Gene_Symbol=- Ring-finger protein 213 | Unconfirmed | 1.32 | 591 | 35.64 | 3.52E−01 |
| Gene_Symbol=Sparcl1 SPARC-like protein 1 | Classical | 1.35 | 78 | 16.46 | 2.61E−02 |
| Gene_Symbol=Tsen34 34 kDa protein | Unconfirmed | 1.42 | 38 | 74.36 | 1.26E−02 |
| Gene_Symbol=Tln1 Talin-1 | Exosome | 2.02 | 270 | 35.22 | 5.78E−05 |
| Gene_Symbol=Tubb2c Tubulin beta-2C chain | Unconfirmed | 1.60 | 53 | 60.45 | 4.91E−02 |
| Gene_Symbol=Hdgf 22 kDa protein | Classical | 1.34 | 22 | 24.26 | 1.69E−03 |
| Gene_Symbol=Lrpprc Leucine-rich PPR motif-containing protein | Exosome | 1.39 | 130 | 35.34 | 2.41E−02 |
| Gene_Symbol=Ywhae 14-3-3 protein epsilon | Exosome | 1.31 | 31 | 80.39 | 3.19E−02 |
| Gene_Symbol=Trim28 Isoform 2 of Transcription intermediary factor 1-beta | Unconfirmed | 1.40 | 100 | 67.00 | 2.68E−02 |
| Gene_Symbol=Tagln2 Transgelin-2 | Exosome | 1.31 | 22 | 85.43 | 6.46E−03 |
Abbreviations: 4α-PDD, 4α-phorbol 12,13-didecanoate; siRNA, small interfering RNA.