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. 2017 May 22;6(5):e341. doi: 10.1038/oncsis.2017.41

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Copyright © 2017 The Author(s)

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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AKT inhibition by 3-AWA co-ordinates Par-4/JNK intrication in p21 downmodulation. (a) PC-3 cells were transiently transfected with siRNA control or Par-4 siRNA and then treated with 3-AWA and/or Salubrinal for 24 h. The expression of the indicated proteins was analyzed through western blotting. (b) PC-3 cells were transiently transfected with GFP or GFP-Par-4 and then treated with 3-AWA and/or Thapsigargin for the set time point. The lysates were prepared and subjected to western blotting for the expressional analysis of Par-4, p-JNK, JNK and p21. (c) Immunoblotting analysis of Par-4, p-AKT, AKT and p21 was carried out in PC-3 cells, transiently transfected with GFP or GFP-Par-4, then treated with 3-AWA and/or Thapsigargin or an AKT inhibitor AT7867. (d) Lysates prepared from DU-145 cells, transiently transfected with GFP or GFP-Par-4 following treatment with designated concentrations 3-AWA and/or Thapsigargin or AT7867 for the indicated time point, were subjected to western blotting for the detection of Par-4, p-AKT, AKT, p-JNK, JNK, GRP78 and p21. β-Actin was used as loading control and the data shown are the representative of three independent experiments.