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. Author manuscript; available in PMC: 2017 Jul 24.
Published in final edited form as: J Immunol. 2016 Jan 20;196(4):1741–1752. doi: 10.4049/jimmunol.1501272

Figure 1. Active VCA0848 produces significant amounts of c-di-GMP in mice.

Figure 1

Male 6–8 weeks old BALB/c WT mice were retro-orbitally i.v. injected with 2×109 vps/mouse of AdVCA0848 (n=3); or 2×1011 vps/mouse of AdVCA0848mut (n=3) or AdVCA0848 (n=3). As a control not injected (naïves) mice (n=2) were included. At 24 hpi mice were sacrificed and liver samples were collected, and immediately snap frozen in liquid nitrogen. 20 mg of liver samples were used for c-di-GMP extraction as described in methods section. C-di-GMP production measurements were performed using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). Bars represent mean ± SD from different groups. Statistical analysis was completed using One Way ANOVA followed by a Student-Newman-Keuls post-hoc test. A value of p<0.05 was deemed statistically significant. “bd”, below detection.