Table 1.

Relative levels of IbpA and IbpB after two hours of α-glucosidase production at various temperatures with and without ibpAB overexpression from plasmid

	Temperature	wildtype	ibpAB++
IbpAa	42°C	3.3 ± 1.3	84 ± 9.5
	37°C	5.0	50
	30°C	1.6	19
IbpB	42°C	1 ± 0.3	12 ± 1.3
	37°C	1.4	7
	30°C	0.7	3

^a The level of IbpA and IbpB was estimated by densitometry of Coomassie-stained two-dimensional gels. Spot volumes were normalised using three spots as internal standards (indicated by circles in Fig. 2) and are given relative to the value of chromosomally encoded IbpB at 42°C.