Figure 2.

Supra- and infrapatellar ASC proliferative activity. (a) Colony-forming unit (CFU) assay was performed from infra and suprapatellar SVF from four different patients in the presence of 10% human serum (HS) or 10% fetal bovine serum- (FBS-) containing medium for 10 days. The generated colonies were counted after Giemsa staining (right panel). The mean ± SD of the total number of colonies at each condition of all tested samples was shown. ^∗P < 0.05 at the indicated comparisons. (b) 10⁴ cells at passage three of every sample, suprapatellar (blue line)- and infrapatellar (red line)-derived ASC were allowed to grow in multiple wells in the presence of 10% HS or 10% FBS and quantified every day up to 10 days. The mean ± SD of the total number of cells at every time point was represented. ^∗P < 0.05 versus infrapatellar. (c) Representative images after TEM acquisition of suprapatellar ASC growth in HS (left panels) or FBS (right panels) are shown. White arrows: inclusion bodies; black arrows: intermediate filaments.