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. 2017 Jul 9;2017:4758930. doi: 10.1155/2017/4758930

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Copyright © 2017 Ignacio Muñoz-Criado et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Multilineage potential of suprapatellar- and infrapatellar-derived ASC. All directed differentiation processes were induced from both samples, suprapatellar- and infrapatellar-derived ASC at passages after 4. To quantify the efficiency of cell differentiation, specific staining was employed for each process: Oil Red O staining for adipogenesis, Alizarin red for osteogenesis and Alcian blue for chondrogenesis, ^∗P < 0.05 versus infrapatellar. In addition, immunodetection of Connexin 43 (red; osteocyte marker) and phalloidin (green; cytoskeletal marker) was performed to evaluate osteocyte maturation. Sox9 (green) counterstained with DAPI (blue, nucleus) stained newly generated chondrocytes.