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Effects of Gal-3 knockdown on cell proliferation and survival. (a) Proliferation rate in different MSC lines, evaluated by 3H-thymidine incorporation assay. ∗P < 0.05, compared to the other groups. (b) Apoptosis analysis by Annexin V/7-AAD assay, comparing the rate of cells undergoing apoptosis after incubation with 10 μM H2O2. MSC-WT = wild-type MSC; MSC-Ctrl = MSC transduced with a nontargeting shRNA vector; MSC-Gal3KD = Gal-3 knockdown MSC. ∗P < 0.05.
