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. 2017 Jul 10;2017:3282656. doi: 10.1155/2017/3282656

Figure 4.

Figure 4

Gal-3 knockdown MSC exhibit defective migration and displacement in vitro. Migration was evaluated by the wound healing assay. Phase contrast representative images showing scratch area at day 0 for wild-type MSC (a) and Gal-3 knockdown MSC (c), and at day 3 for wild-type MSC (b) and Gal-3 knockdown MSC (d). (e) Gap distance was evaluated at 72 h and compared to time 0. ∗∗∗P < 0.001. (f–h) Mean square displacements were obtained by individually tracked cells at various time points, from the time of the first position, until the end of the overnight incubation of MSC-WT (d), MSC-Ctrl (e), and MSC-Gal3KD (f). MSC-WT = wild-type MSC; MSC-Ctrl = MSC transduced with a nontargeting shRNA vector; MSC-Gal3KD = Gal-3 knockdown MSC.