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. 2017 Jul 24;8:1361. doi: 10.3389/fmicb.2017.01361

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Copyright © 2017 Morawe, Hoeke, Wissenbach, Lentendu, Wubet, Kröber and Kolb.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Species-Level resolution of the Beijerinckiaceae-affiliated phylotype OTU_16S438. Species-level phylotypes of the Beijerinckiaceae-affiliated OTU_16S438 (, phylotype A to I based on species level similarity cut-off) and their putative substrate utilization. Outgroup, 16S rRNA sequence of Methylococcus capsulatus BATH. Bootstrap were based on 1000 replicated calculations. The trees was calculated with the neighborjoining method. Dots at the nodes, congruent nodes with trees caculated with the maximum likelihood and maximum parsimony method (●, true for three methods; , true for two methods). If known the isolation origin of each sequence is given in brackets. Accession numbers are given in squared brackets. The bar indicates 5 changes per nucleotide. ‘Sub-SIP,’ substrate SIP experiment; ‘pH-SIP,’ pH shift SIP experiment; cross, additional [¹²C]-methanol supplementation in substrate treatments of the substrate SIP experiment.

Inline graphic — Species-Level resolution of the Beijerinckiaceae-affiliated phylotype OTU_16S438. Species-level phylotypes of the Beijerinckiaceae-affiliated OTU_16S438 (, phylotype A to I based on species level similarity cut-off) and their putative substrate utilization. Outgroup, 16S rRNA sequence of Methylococcus capsulatus BATH. Bootstrap were based on 1000 replicated calculations. The trees was calculated with the neighborjoining method. Dots at the nodes, congruent nodes with trees caculated with the maximum likelihood and maximum parsimony method (●, true for three methods; , true for two methods). If known the isolation origin of each sequence is given in brackets. Accession numbers are given in squared brackets. The bar indicates 5 changes per nucleotide. ‘Sub-SIP,’ substrate SIP experiment; ‘pH-SIP,’ pH shift SIP experiment; cross, additional [¹²C]-methanol supplementation in substrate treatments of the substrate SIP experiment.