Fig. 1.

CYP1B1 promotes the protein expression levels of β-catenin and cyclin D1 in HeLa cells. (A) RT-PCR analyses. HeLa cells were transfected with pcDNA3.1(zeo)-CYP1B1 (5 μg) or CYP1B1 specific siRNA (40 nM) for 48 hr. After RNA isolation, gene amplification of CYP1B1 was conducted. GAPDH mRNA was determined as a control. (B) Quantitative PCR analyses. Total RNA was isolated and qPCR for CYP1B1 was performed in triplicate. Data were normalized to the GAPDH value. The data represent mean ± SD. *p≤0.05. (C) Western blot analyses. The cells were transfected with pcDNA 3.1(zeo)-CYP1B1 (5 μg) or CYP1B1 specific siRNA (40 nM). After stabilization for 48 hr, total protein was isolated from cell lysates, and the protein expression levels of CYP1B1, β-catenin, and cyclin D1 were measured. β-Actin was used as an internal loading control. Empty vector (pcDNA3.1(zeo))-transfected group was represented as control and CYP1B1-overexpressed group was represented as CYP1B1 in figures. Scrambled siRNA-transfected group was represented as siCON while CYP1B1 specific siRNA-transfected group was represented as si1B1 in figures.