Table I. List of conditions used in in vitro fate analysis and chemotactic assay.

Condition	Host Species	Concentration or Dilution Used	Manufacturer	Catalogue Number
Purified C1q (C1q[PMN])	Human serum	28 ng/ml	My Biosource	MBS143105
Purified C1q (C1q[Mϕ])	Human serum	400 ng/ml	My Biosource	MBS143105
Purified C3a (C3a[PMN])	Human	18.5 ng/ml	Quidel	Component of kit A015, predecessor to A031
Purified C3a (C3a[Mϕ])	Human	120 ng/ml	Quidel	Component of kit A015, predecessor to A031
Purified C3a desArg	Human	18.5 ng/ml	Complement Technologies	A119
SDF1α	Human	200 ng/ml	PeproTech	300-28A
αC1q	Goat anti-human	20 μg/ml (chemotaxis); 1.65 μg/ml (fate); 100 μg/ml in vivo	Quidel	A301
αC3α	Mouse anti-human	20 μg/ml (chemotaxis); 1.65 μg/ml (fate); 100 μg/ml in vivo	Millipore	CBL191
αTNF-α	Rabbit anti–TNF-α	2 and 6 μg/ml	Millipore	AB2148P
αSDF1α	Mouse anti-SDF1α	100 μg/ml	Millipore	MABC184
C1q IgG control	Goat IgG	20 μg/ml in vitro and 2000 μg/ml in vivo	Jackson ImmunoResearch and R&D Systems	005-000-005
C3a IgG control	Mouse IgG	20 μg/ml in vitro and 2000 μg/ml in vivo	Jackson ImmunoResearch and R&D Systems	015-000-003
LY294002	N/A	50 μM dissolved in DMSO	Life Technologies	PHZ1144
PD98059	N/A	10 μM dissolved in DMSO	Life Technologies	PHZ1164
DMSO	N/A	1:1000	Sigma-Aldrich	D26650

N/A, not available.