Table II. List of Abs used for immunocyto/histochemical analyses and Western blots.
| Ab | Host | Dilution of Primary Ab (In Vivo) | Dilution of Primary Ab (In Vitro) | Manufacturer of Primary Ab | Specificity of Primary Ab | Secondary Ab (In Vitro and In Vivo) |
|---|---|---|---|---|---|---|
| BrdU | Rat | N/A | 1:100 | Serotec | Proliferating cells | Alexa Fluor donkey anti-rat Fab fragment 488 (1:5–10,000) |
| β-Tubulin III | Rabbit | 1:5000 | 1:100 | Covance | Neurons | Biotinylated or Alexa Fluor 488–conjugated donkey anti-rabbit Fab fragment (1:5–10,0000) |
| CC1 | Mouse | 1:4000 | N/A | Calbiochem | Mature oligodendrocytes | Biotinylated donkey anti-mouse Fab fragment (1:10,000) |
| CC3 | Rabbit | N/A | 1:100 | Cell Signaling Technology | Apoptotic cells | Alexa Fluor donkey anti-rabbit IgG 555 (1:5,000) |
| Fibronectin | Rabbit | 1:500 | N/A | Sigma-Aldrich | Fibronectin-containing lesion | Biotinylated donkey anti-rabbit Fab fragment (1:10,000) |
| GFAP | Rabbit | 1:60,000 | 1:100 | Dako | GFAP-positive astrocytes | Biotinylated or Alexa Fluor 555–conjugated donkey anti-rabbit Fab fragment (1:10,000) |
| Olig-2 | Goat | 1:100 | 1:100 | R&D Systems | Oligodendrocyte progenitor cells | Biotinylated or Alexa Fluor 488–conjugated donkey anti-goat Fab fragment (1:10,000) |
| SC121 | Mouse | 1:3000 | N/A | StemCells | Human cytoplasm | Biotinylated donkey anti-mouse Fab fragment (1:10,000) |
| SC123 | Mouse | 1:3000 | N/A | StemCells | Human-positive astrocytes | Biotinylated donkey anti-mouse Fab fragment (1:10,000) |
| Sox2 | Goat | 1:200 | N/A | Santa Cruz Biotechnology | Sox2-positive progenitor cells | Alexa Fluor donkey anti-goat 488 (1:200) |
| GAPDH | Rabbit | 1:1000 | N/A | Cell Signaling | Housekeeping | ECL donkey anti-mouse Fab fragment (1:10,000) |
| C3 | Goat | 1:200 | N/A | Quidel | Human and mouse C3 | ECL donkey anti-goat IgG, HRP-linked whole Ab (1:10,0000) |
| C1q | Goat | 1:200 | N/A | Quidel | Human and mouse C1q | ECL donkey anti-goat IgG, HRP-linked whole Ab (1:10,0000) |
| β-Actin | Mouse | 1:1000 | N/A | Sigma-Aldrich | β-actin | ECL donkey anti-mouse IgG, HRP-linked whole Ab (1:10,0000) |
| p-ERK | Rabbit | 1:1000 | N/A | Cell Signaling Technology | Phosphorylated ERK | ECL donkey anti-rabbit IgG, HRP-linked whole Ab (1:10,0000) |
| p-AKT | Rabbit | 1:1000 | N/A | Cell Signaling Technology | Phosphorylated AKT | ECL donkey anti-rabbit IgG, HRP-linked whole Ab (1:10,0000) |
N/A, not applicable.