FIGURE 2.

Cav1 regulates CD8 T cell responses to Ag. (A) The frequency of CD69⁺ cells among Cav1-WT and Cav1-KO OT-1 CD8 T cells stimulated for 3 h with a titration of N4, T4, or G4 peptides. (B and C) Cav1-WT and Cav1-KO OT-1 CD8 T cells were stimulated and supernatants were assayed at 24 h for total IL-2 (B) and TNF-α (C) by ELISA. (D) Proliferation at 72 h of CTV-labeled OT-1 T cells is represented by the division index calculated using FlowJo software. Data are representative of two independent experiments, each performed on three mice per group. Error bars represent SD. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 (Student t test, corrected for multiple comparisons using the Holm–Sidak method).