FIGURE 3.

Impaired recruitment of F-actin and CD11a to the IS in the absence of Cav1. ImageStream analysis assessing conjugate formation of Cav1-WT or Cav1-KO OT-1 CD8 T cells with N4-pulsed APCs for the indicated times (0–20 min) is shown. Original magnification ×60. Conjugates were fixed, permeabilized, and stained for BODIPY-Fl (F-actin, green) and CD11a (yellow). (A) The four rows show representative cells at 20 min, with distribution of BODIPY-Fl and CD11a within the synapse formed between T cells (purple) and APCs (red) shown in merge columns 4 and 5, respectively. Graphs of the internalization method, previously described in (35), for Cav1-WT and Cav1-KO conjugates stained with (B) BODIPY-Fl and (C) CD11a are representative of three independent experiments.