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Western blot analysis of myocardial estrogen receptor‐α (ER‐α) (A) and estrogen receptor‐β (ER‐β) (B) levels showing increased ER‐α in male WT hearts with no changes observed in response to iron overload; n=6 per group; *P<0.05 for effect of ovariectomy. HJVKO indicates hemojuvelin‐null; OVX, ovariectomy; R.R., relative ratio; WT, wild‐type.
