Table 3.
LV Parameters Analyzed by 2D Echocardiography in cfms‐icre;Wls fl/fl and Wls fl/fl Mice at Baseline and Days 1, 7, and 30 After MI
| Days After MI | Wls fl/fl (n=15) | cfms‐icre;Wls fl/fl (n=10) | P (Repeated‐Measures ANOVA) | |||
|---|---|---|---|---|---|---|
| Wls−/− Effect | Time Effect | Interaction | ||||
| Ejection fraction, % | Baseline | 52.14±2.39 | 61.65±3.22 | 0.0011 | <0.0001 | 0.2944 |
| 1* | 38.05±3.43 | 50.26±3.16 | ||||
| 7 | 41.58±2.16 | 49.66±3.27 | ||||
| 30‡ | 37.88±2.23 | 54.64±4.23 | ||||
| LV diastolic area, mm2 | Baseline | 12.38±0.41 | 11.38±0.54 | 0.4975 | <0.0001 | 0.2069 |
| 1 | 10.47±0.56 | 9.94±0.95 | ||||
| 7 | 11.24±0.59 | 12.01±0.64 | ||||
| 30 | 13.14±0.57 | 12.02±0.80 | ||||
| LV systolic area, mm2 | Baseline | 7.08±0.36 | 5.78±0.49 | 0.0547 | 0.0249 | 0.2552 |
| 1 | 7.03±0.56 | 6.03±0.69 | ||||
| 7 | 7.26±0.47 | 6.76±0.60 | ||||
| 30* | 8.72±0.57 | 6.54±0.74 | ||||
| Fractional shortening, % | Baseline | 26.63±1.56 | 33.01±2.11 | 0.0007 | <0.0001 | 0.3361 |
| 1* | 18.36±1.93 | 25.15±1.88 | ||||
| 7 | 20.14±1.19 | 25.11±1.97 | ||||
| 30‡ | 18.28±1.21 | 28.63±2.88 | ||||
| Posterior wall thickness, diastole, mm | Baseline | 0.75±0.01 | 0.79±0.02 | 0.5700 | 0.1818 | 0.1680 |
| 1 | 0.78±0.02 | 0.82±0.04 | ||||
| 7 | 0.81±0.02 | 0.76±0.01 | ||||
| 30 | 0.81±0.01 | 0.82±0.02 | ||||
| Posterior wall thickness, systole, mm | Baseline | 1.03±0.03 | 1.11±0.03 | 0.0515 | 0.6576 | 0.0336 |
| 1 | 1.01±0.03 | 1.13±0.04 | ||||
| 7 | 1.06±0.03 | 1.02±0.03 | ||||
| 30* | 1.01±0.02 | 1.16±0.06 | ||||
| FAC, % | Baseline | 42.93±1.99 | 49.60±2.75 | 0.0041 | 0.0004 | 0.3843 |
| 1 | 34.05±2.54 | 39.99±3.06 | ||||
| 7 | 35.72±1.96 | 44.27±2.95 | ||||
| 30† | 33.95±2.20 | 46.61±3.61 | ||||
| LV diastolic dimension, mm | Baseline | 3.99±0.06 | 3.90±0.10 | 0.4113 | <0.0001 | 0.0504 |
| 1 | 3.69±0.10 | 3.53±0.16 | ||||
| 7 | 3.85±0.11 | 4.04±0.11 | ||||
| 30 | 4.33±0.09 | 4.03±0.11 | ||||
| LV systolic dimension, mm | Baseline | 2.93±0.08 | 2.62±0.13 | 0.2108 | <0.0001 | 0.1092 |
| 1 | 3.03±0.14 | 2.65±0.16 | ||||
| 7 | 3.09±0.11 | 3.03±0.14 | ||||
| 30 | 3.55±0.11 | 2.90±0.18 | ||||
| Anterior wall thickness diastole, mm | Baseline | 0.88±0.02 | 0.88±0.03 | 0.6982 | <0.0001 | 0.6849 |
| 1 | 1.08±0.04 | 1.12±0.05 | ||||
| 7 | 0.96±0.05 | 0.92±0.04 | ||||
| 30 | 0.92±0.03 | 0.97±0.03 | ||||
| Anterior wall thickness systole, mm | Baseline | 1.18±0.03 | 1.28±0.06 | 0.1618 | 0.3164 | 0.8124 |
| 1 | 1.23±0.06 | 1.31±0.06 | ||||
| 7 | 1.16±0.06 | 1.17±0.06 | ||||
| 30 | 1.13±0.03 | 1.24±0.07 | ||||
| LV mass, mg | Baseline | 121.81±4.22 | 122.12±7.50 | 0.7138 | <0.0001 | 0.5013 |
| 1 | 130.63±8.93 | 126.91±6.27 | ||||
| 7 | 126.50±4.47 | 128.80±5.22 | ||||
| 30 | 150.67±8.04 | 140.08±4.48 | ||||
| LV volume, diastole, mm3 | Baseline | 70.15±2.55 | 66.59±4.03 | 0.4200 | <0.0001 | 0.0399 |
| 1 | 58.93±3.76 | 53.52±5.48 | ||||
| 7 | 65.39±4.58 | 72.46±4.84 | ||||
| 30 | 85.53±4.53 | 72.32±4.94 | ||||
| LV volume, systole, mm3 | Baseline | 33.69±2.19 | 26.22±3.29 | 0.0321 | <0.0001 | 0.0333 |
| 1 | 37.89±3.88 | 27.39±3.81 | ||||
| 7 | 38.84±3.65 | 37.31±4.54 | ||||
| 30† | 54.02±4.57 | 34.32±5.16 | ||||
Results are presented as mean±SEM. Statistical analysis: 2‐way repeated‐measures analysis of variance with Bonferroni posttest. 2D indicates 2‐dimensional; cfms, colony‐stimulating factor 1 receptor; FAC, fractional area change; LV, left ventricle; MI, myocardial infarction; Wls, Wntless.
*P<0.05, † P<0.01, ‡ P<0.001 vs Wls fl/fl.