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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: J Neuroendocrinol. 2017 Jun;29(6):10.1111/jne.12491. doi: 10.1111/jne.12491

Figure 2.

Figure 2

Neuropeptide coexpression patterns in the arcuate nucleus of ovariectomized (OVX) adult female rhesus macaques. Quantification of coexpression in cell bodies, when visible, and axon varicosities by immunohistochemistry, was performed for arcuate neuropeptides regulating reproduction and appetite. Kisspeptin showed only a 7% coexpression with dynorphin (top panel) in cell bodies but 54% colocalization in axon varicosities. CART-ir was present in 21% of kisspeptin-ir positive cell bodies and 41% of axon varicosities (second panel). CART axon varicosities showed a high level of colocalization of 97% with NPY (third panel), although no NPY-ir was observed in cell bodies; therefore, coexpression in cell bodies was not quantified. CART showed extremely low colocalization of only 4% of axon varicosities with α-MSH (fourth panel) while no cell bodies showed immunoreactivity for both neuropeptides. NPY-ir was also present in 87% of AgRP-ir axon varicosities (fifth panel). Images are approximately 150 x 150 μm.