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Arcuate AgRP and NPY fiber density by immunohistochemisty in prepubertal and OVX adult female rhesus macaques. Quantification of AgRP-ir (left panels), NPY-ir (middle panels) and colocalized AgRP/NPY-ir (right panels) showed significantly lower levels of AgRP-ir and AgRP/NPY-ir in OVX adult animals (bottom panels) compared to prepubertal animals (top panels). N=5, * p<0.05 by t-test and images are approximately 150 x 150 μm.
