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. Author manuscript; available in PMC: 2018 May 17.
Published in final edited form as: Neuron. 2017 May 17;94(4):759–773.e8. doi: 10.1016/j.neuron.2017.04.043

Figure 5. Effects of serum exposure on microglial gene expression.

Figure 5

(A–C) Volcano plots summarizing changes in microglial gene expression (P21, 8 div in TIC) after 1, 3, or 5 days of serum exposure as measured by RNA-seq. Values in the upper corners denote the number of genes upregulated (red) or downregulated (blue) after serum exposure at each time point passing a 4-fold cutoff at P<0.01. Individual genes showing pronounced changes are labeled. (D) KEGG pathways enriched after serum exposure among upregulated (red, left) or downregulated (blue, right) genes. Enrichment was measured using GSEA, and the false-discovery-rate q-value (FDR) is plotted, with larger bars indicating higher confidence of enrichment. Pathways involving proliferation, amino acid metabolism, and complement are upregulated after serum exposure, whereas cytokine pathways and lipid-modifying pathways are downregulated.