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. Author manuscript; available in PMC: 2018 May 23.
Published in final edited form as: Cell Rep. 2017 May 23;19(8):1602–1613. doi: 10.1016/j.celrep.2017.05.004

Figure 3. Hand2-deficient AVC endocardial cells fail to initiate EMT and mesenchymal cell migration.

Figure 3

(A) Smooth muscle actin (red) and F-actin (green) distribution in cells that have migrated into the matrix from AVC explants of wild-type and Hand2-deficient embryos after 72hrs in culture. Scale bars: 100μm. Bottom panels show cells in proximity to the explant (i and iii) and at the far edge of migration (ii and iv). Scale bars: 20μm. (B) Quantification of the numbers of cells that migrated into the matrix from wild-type (n=10) and Hand2-deficient AVC explants (n=7). The mean ± SD (p=0.0001, Mann-Whitney test) and all individual data points are shown.