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. Author manuscript; available in PMC: 2018 May 23.
Published in final edited form as: Cell Rep. 2017 May 23;19(8):1602–1613. doi: 10.1016/j.celrep.2017.05.004

Figure 5. Whole mount in situ hybridization reveals spatial changes in some of the differentially expressed HAND2 target genes.

Figure 5

(A, B) Unaltered expression of Bmp2 (panel A) and Hey2 (panel B) indicates that the AVC domain is established correctly in Hand2-deficient hearts. (C, D) Ectopic expression of the Hey1 (panel C) and Hhex transcriptional regulators (panel D) in the mutant AVC corroborated their up-regulation detected by RNA-Seq analysis. (E-F) Loss of Twist1 (panel E), Msx1 (panel F), Sox9 (panel G) and Has2 (panel H) from the mutant AVC is in agreement with their transcriptional down-regulation detected by RNA-Seq analysis. (I) Alcian blue staining of glycosaminoglycans shows the reduced deposition of extra-cellular matrix in the cardiac jelly of Hand2-deficient mouse embryos. Right panels show the enlargements indicated by frames in the left panels. Gene names in black: unaltered, red: increased, blue: reduced transcript levels as determined by RNA-Seq analysis (Figure 4). Scale bars: 100μm. See also Figure S5.