Skip to main content
. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Clin Chem. 2017 Apr 10;63(6):1083–1093. doi: 10.1373/clinchem.2016.262253

Figure 3.

Figure 3

Use of zonal elution-based competition studies in HPAC to examine the direct competition of a drug or solute with an injected probe for specific binding sites on an immobilized protein, as illustrated by the image on the left for the competition of glimepiride with L-tryptophan at Sudlow site II on columns that contained normal HSA (●) or two glycated preparations of this protein (■,▲). The image on the right shows of modification in glycated HSA and the location of the two major drug binding sites on this protein, Sudlow sites I and II. Reproduced from Refs. (11,24) with permission from Elsevier.