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. Author manuscript; available in PMC: 2017 Jul 24.
Published in final edited form as: J Immune Based Ther Vaccines Antimicrob. 2013 Jul 1;2(3):29–38. doi: 10.4236/jibtva.2013.23004

Figure 5. Effect of HLA-DR4-derived peptides on cytokine production.

Figure 5

PBMCs from 3 females (A and B) and 6 males (C and D) were incubated for 8 days with the peptides at 100 µg/ml with or without TH17-polarizing cytokines. Concentrations of IL-17A (A and C) and IFNγ (B and D) in supernatants were quantified by ELISA. Data represent mean ± SEM of concentration of cytokines (pg/ml) modeled by linear mixed model analysis (SPSS, IBM), using a gender stratified data file and considering main effects of SE status and treatment with TH17 polarizing cytokines or peptides. The basal value reflects the average cytokine concentration obtained in a condition were all the parameters are set to zero as discussed in the legend to Figure 2. **, p<0.01; ***, p<0.001.