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MicroRNA (miR)‐181‐sponge‐expressing cells have upregulated PTEN expression. A, Western blot analysis of phosphatase and tensin homolog (PTEN), phospho‐Akt Ser 473, and total Akt expression in the miR‐181‐sponge‐H9c2 and scramble‐sponge–expressing cells. Lysates were probed with the indicated antibodies. α‐Tubulin is a loading control. B and C, Band‐densitometry is indicated. B, *P<0.05 vs EGFP‐scramble‐H9c2. C, *P<0.05 vs scramble (n=4).
