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. 2017 Feb 27;6(3):e004694. doi: 10.1161/JAHA.116.004694

Figure 4.

Figure 4

MicroRNA (miR)‐181‐sponge protects cells against oxidative stress induced by doxyrubicin. A, Analysis of doxorubicin (DOX) treatment on cellular viability. Lactic acid dehydrogenase release, in scramble‐sequence (Scr)‐ and miR‐181‐sponge (Sp)‐expressing cells treated with 10 μmol/L with/without LY294002 (LY) for 48 hours. *P<0.05 vs corresponding scramble (n=8). B, Western blot analysis of pAkt Thr 308, and total Akt expression in the scramble‐sponge (Scr)‐ and miR‐181‐sponge (Sp)‐expressing cells pretreated with insulin (200 nmol/L) for 5 minutes after 16 hours of serum starvation. We have used miR‐181‐sponge untreated group (Sp‐Ins) as a negative control for insulin treatment. Lysates were probed with the indicated antibodies. Total Akt was used as a normalization control for pAkt (Thr 308). We also used α‐tubulin to normalize total Akt expression. *P<0.05 vs scramble (n=4).