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. 2017 Mar 14;6(3):e005041. doi: 10.1161/JAHA.116.005041

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© 2017 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley Blackwell.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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VIC proliferation and apoptosis decrease after culture in FIB‐Coll. A, Quantification of the percentage of proliferating cells after 8 hours of incubation with EdU. B, Evaluation of apoptosis levels through a caspase 3/7 activity assay. *P<0.01 compared with control VICs at the same time point. ^{^} P<0.01 compared with day 4 VICs cultured in the same condition. ^# P<0.01 for comparisons indicated on graph. n=6 to 8. FIB indicates fibroblast media formulation; FIB‐Coll, fibroblast media formulation with culture on collagen coatings; VIC, valvular interstitial cell.