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. 2017 May 3;6(5):e005771. doi: 10.1161/JAHA.117.005771

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© 2017 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Linear regression of standard curve obtained from serial dilutions of the pLenti6.2‐GW/EmGFP plasmid (3—3×10⁶ copy number) assayed in duplicate by qPCR. R ² and equation of linear regression are described in the intersection point of the chart. Ct indicates threshold cycle; GFP, green fluorescent protein; qPCR, quantitative real‐time polymerase chain reaction.