Table 1.
Primer Sequences for Quantitative RT‐PCR
| Gene | Oligo Name | Oligo Sequence (5′ to 3′) |
|---|---|---|
| CSF2 (NM_214118.2) | GM‐CSF Forward | GGATTGCTCCCACTGACAGA |
| GM‐CSF Reverse | GCAAGTCTGTGCCCCATTAC | |
| IL‐10 (NM_214041.1) | IL10 Forward | GGGAAACCCTGCTGTACCTC |
| IL10 Reverse | TGAACACCATAGGGCACACC | |
| SDF‐1α (NM_001009580.1) | SDF‐1α Forward | CCGAACTGTGCCCTTCAGAT |
| SDF‐1α Reverse | ATAAACATCCCGCCGTCCTC | |
| HGF (XM_013979787.1) | HGF Forward | TGCTCCTCCCTTCCCTACTC |
| HGF Reverse | CCCGATAGCTCGAAGGCAAA | |
| VEGF (AF318502.1) | VEGF Forward | CCACGAAGTGGTGAAGTTCATG |
| VEGF Reverse | CCACCAGGGTCTCGATTGG | |
| MET (NM_001038008) | HGFR Forward | CAGAAGAGCAAGGGAGAGTGT |
| HGFR Reverse | GGGCACCCAGGTAAATGTGAT | |
| MAPK3 (XM_013991188.1) | ERK‐1 Forward | GGCTCATCCTTACCTGGAGC |
| ERK‐1 Reverse | AGATGAGTTCCTTCAGCCGC | |
| SFRP2 (NM_001244395.1) | SFRP‐2 Forward | CCCGACTTCTCCTACAAGCG |
| SFRP‐2 Reverse | GCCGCATGTTCTGGTACTCT | |
| ACTB (XM_003124280.4) | β‐Actin Forward | GTGACAGCAGTCGGTTGGAT |
| β‐Actin Reverse | TTTTGGGAAGGCAGGGACTT |
ACTB indicates actin beta; CSF, colony‐stimulating factor; HGF, hepatocyte growth factor; GM‐CSF, granulocyte‐macrophage colony‐stimulating factor; IL‐10, interleukin‐10; MAPK3, mitogen‐activated protein kinase 3; MET, MET proto‐oncogene; RT‐PCR, reverse transcription polymerase chain reaction; SDF‐1α, stromal‐derived factor‐1α; SFRP2, secreted frizzled‐related protein 2.