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. 2017 Apr 25;9(5):781–791. doi: 10.1080/19420862.2017.1320008

Figure 4.

Figure 4.

Effect of incubation time and donor/acceptor chamber pH on transcytosis of IgG mutants by human FcRn-over-expressing cells. The application of 5 h incubation time and a pH gradient of 6.0/7.4 differentiated the various molecules best. The fluxes were normalized to the flux of chicken IgY. Data is shown as mean ± SD; n  =  3 (separate wells).