Table 1.

In vitro flux of IgG mutants across human and rat FcRn MDCK cells at pH 6.0/7.4.

Tested antibody	Flux across rat FcRn cells	Flux across human FcRn cells
IgY	1.0 ± 0.11	1.0 ± 0.03
AAA IgG	1.34 ± 0.26	0.70 ± 0.02
Wt IgG	2.72 ± 0.31	1.95 ± 0.12
YTE IgG	2.41 ± 0.38	3.11 ± 0.06

Only the YTE mutant showed a species difference in FcRn interaction leading to enhanced flux across human FcRn, but Wt-IgG-like flux across rat FcRn cells. The fluxes were normalized to the IgY flux obtained in the same experiment. Data is shown as mean ± SD; n  =  3 to 6 (separate wells).