Table 7.

In vitro studies for anti-amyloid β mAb.

Species	Study	Findings
Human	Cytokine release	Cytokine release assay in whole human blood (IL-1ra, IL-1b, IL-6, hTNFa, IL-8) was positive for IL-8 release at ≥ 10 µg/mL.
Human	PBMC binding	No PBMC staining at 10 or 30 µg/mL.
Human, cynomolgus monkey, Tg mice (3 donors each)	Tissue cross-reactivity with FITC-labeled Ab (GLP)	No noticeable binding.
Human and cynomolgus monkey	Platelet binding (FACS)	No binding to platelets in human blood (Fc mutation and wt alike). No binding to CD41+ platelets in cynomolgus blood (Fc mutation and wt alike). Some binding to CD41- “platelet-like” cells in cynomolgus blood (Fc mutation and wt alike).
Human, cynomolgus monkey, mouse and rat	Serum binding (ELISA)	Concentration-dependent binding to serum component of species tested (Fc mutation and wt alike). No binding of murine Ab to any species. Binding to serum component is not CDR/target mediated; suggests that binding is not Fc mediated.