Skip to main content
. Author manuscript; available in PMC: 2017 Nov 10.
Published in final edited form as: Nature. 2017 May 10;546(7658):370–375. doi: 10.1038/nature22403

Extended Data Figure 2. Pluritest scores in the two culture conditions.

Extended Data Figure 2

(a-c) Comparison of PluriTest novelty score versus pluripotency score for the 711 lines generated. Lines grown on feeder-free conditions (E8 media) scored systematically lower than Feeder-dependent lines (P = 1.62x10-43 t-test, for pluripotency score). We note that, while we cannot rule out that Feeder-free lines are less pluripotent, Feeder-free conditions are not well represented in the PluriTest training dataset, which may explain this result (of the 204 ESC/IPSC lines in the pluriTest paper that have media metadata available, none were on E8 and only 37 were on a variety of other feeder free formulations such as MTSER). (d) Despite lower pluripotency scores, lines grown on Feeder-free conditions have higher fractions of cells expressing canonical protein markers of pluripotency.